Journal of Plant Biotechnology

Table. 2. Table 2

Primers and restriction enzymes used to develop S. chacoense specific markers

Marker name	Region	S^a	Primer sequence	Size (bp)^b	RE^c
SC3_InDel_11	rps4 (Intragenic)	F	TTGTATCTTTATCCCGGAGC	300	as
SC3_InDel_11	rps4 (Intragenic)	R	GTTGTTAGTAAAATGTTGTTAG	300	as
SC3_InDel_16	accD (Intragenic)	F	CTTTGTTCCGTGTTGAAATA	334	as
SC3_InDel_16	accD (Intragenic)	R	AAGCATAGAAATCCAGTGTT	334	as
SC3_SNP_7	rpoB (Intragenic)	F	CTAGCAGACTCCCTGCTAAC	397	MboII
SC3_SNP_7	rpoB (Intragenic)	R	GTGAAAGAATCTTCGTATGC	397	MboII
SC3_SNP_15	accD (Intragenic)	F	TATAGGAGAGGACAAATCTC	406	MaeI
SC3_SNP_15	accD (Intragenic)	R	TTTCAATAGGACCAAGACTG	406	MaeI

F and R indicate forward and reverse strand of primers.

The expected size of PCR fragments are measured based on the sequence of S. chacoense.

Restriction enzymes generating S. chacoense specific markers. ‘as’ indicates allele specific marker.