
Primers and restriction enzymes used to develop
Marker name | Region | S | Primer sequence | Size (bp) | RE |
---|---|---|---|---|---|
SC3_InDel_11 | F | TTGTATCTTTATCCCGGAGC | 300 | as | |
R | GTTGTTAGTAAAATGTTGTTAG | ||||
SC3_InDel_16 | F | CTTTGTTCCGTGTTGAAATA | 334 | as | |
R | AAGCATAGAAATCCAGTGTT | ||||
SC3_SNP_7 | F | CTAGCAGACTCCCTGCTAAC | 397 | ||
R | GTGAAAGAATCTTCGTATGC | ||||
SC3_SNP_15 | F | TATAGGAGAGGACAAATCTC | 406 | ||
R | TTTCAATAGGACCAAGACTG |
F and R indicate forward and reverse strand of primers.
The expected size of PCR fragments are measured based on the sequence of
Restriction enzymes generating