Fig. 2. Glycoprotein staining of the PAT protein purified from GM zoysiagrass events. M, protein size marker. Size of protein standard (kDa) is shown on the left (lane 1, lane 10); lane 2~3, horseradish peroxidase as a positive control (lane 2, 5 µg; lane 3, 1 µg); lane 4~5, soybean trypsin inhibitor as a negative control (lane 4, 5 µg; lane 5, 1 µg); lane 6~7, purified PAT protein from JG21 (lane 6, 10μg; lane 7, 1 µg); lane 8~9, purified PAT protein from JG21-MS1 (lane 8, 10 µg; lane 9, 1 µg). After the glycoprotein stain, the gel was stained using Comassie brilliant blue.
© 2020 J Plant Biotechnol