Journal of Plant Biotechnology : eISSN 2384-1397 pISSN 1229-2818

Fig. 3.

Download original image
Fig. 3. Effect of RYB on β-catenin proteasomal degradation and determination of the upstream kinases involved in RYB-mediated Wnt/β-catenin degradation in PC-3 cells. (A) PC-3 cells were pretreated with 20 μM MG132 for 2 hours followed by 200 μg/mL RYB for 6 hours. (B) PC-3 cells were pretreated with the GSK-3β inhibitor LiCl (20 mM) for 2 hours followed by RYB (200 μg/mL) for 6 hours. Cell lysates were subjected to SDS-PAGE and western blot was performed using antibody against Wnt/β-catenin. Representative blot (left) and quantification of 3 replicates (right) are provided. Values were normalized to the β-actin loading control. Error bars indicate SD
*p<0.05 compared to the cells without RYB treatment
J Plant Biotechnol 2021;48:106-14 https://doi.org/10.5010/JPB.2021.48.2.106
© 2021 J Plant Biotechnol