Primers and restriction enzymes for generating
| Marker name | Region | S |
Primer sequence | Size (bp) |
RE |
|---|---|---|---|---|---|
| SB2_SNP_3 | F | AATACCATGGTCTAATAATC | 688 | ||
| R | TAATAGTACATCCCAACAGG | ||||
| SB2_SNP_5 | F | AATAACTCCCTTTGGTATTC | 653 | ||
| R | TGTGTTATATCTGGTAATCC | ||||
| SB2_SNP_8 | F | TACGGAATAGAAAGATTCC | 680 | ||
| R | ATTCATGAACTAATGTACTC |
aF and R indicate forward and reverse strand of primers.
bThe expected size of PCR fragments is measured on the basis of the sequence of
cRestriction enzymes generating
