Journal of Plant Biotechnology : eISSN 2384-1397 pISSN 1229-2818

Table. 2.

Table. 2.

Influence of sonication and vacuum infiltration on transformation efficiency in embryo axis explants of chickpea cv. JAKI92181

Sonication duration (s) Vacuum infiltration duration (min) Explants infected, n gus2-Positive explants, mean ± SE3,4
0 - 100 76.5 ± 1.38ef
30 - 100 78.6 ± 1.62de
60 - 100 81.4 ± 1.64cd
90 - 100 73.2 ± 1.68gh
120 - 100 70.4 ± 1.82hi
150 - 100 73.2 ± 1.56gh
90 0 100 81.4 ± 1.62cd
90 5 100 82.6 ± 1.86bc
90 10 100 82.8 ± 1.46bc
90 15 100 84.8 ± 1.58ab
90 20 100 78.8 ± 1.66de
90 25 100 74.6 ± 1.62fg

1The control group consisted of wounded explants precultured for 3 days at a bacterial cell density of 1.0 at OD6005 and an infection duration of 30 min, with 100 µM/l of acetosyringone and 200 mg/l of L-cysteine. Transformation efficiency = (GUS explants/total explants infected) × 100. We used 100 embryo axis explants per treatment, and each treatment was repeated thrice.

2gus: β-glucuronidase.

3SE: standard error.

4Means ± SEs of values of three independent experiments. Values with the same letter within columns are not significantly different according to the DMRT6 at a 5% level.

5OD600: optical density at 600 nm wavelength.

6DMRT: Duncan’s multiple range test.

J Plant Biotechnol 2022;49:61-73 https://doi.org/10.5010/JPB.2022.49.1.061
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