Journal of Plant Biotechnology : eISSN 2384-1397 pISSN 1229-2818

Table. 3.

Table. 3.

Influence of different cultivars of chickpea on Agrobacterium-mediated transformation using embryo axis explants of chickpea cv. JAKI92181

Chickpea genotype Explants infected, n Explants that responded (after 3 subcultures), mean ± SE2,3 gus4-Positive explants, mean ± SE Transformation efficiency (%)5
JAKI9218 100 12.8 ± 0.76a 8.6 ± 0.84a 8.6
JG11 100 10.4 ± 0.68b 7.2 ± 0.78b 7.2
ICCC37 100 9.6 ± 0.86d 5.4 ± 0.92d 5.4
ICCV10 100 7.3 ± 0.82e 4.6 ± 0.83e 4.6
ICCV92944 (JG14) 100 10.2 ± 0.92bc 6.8 ± 0.76bc 6.8
ICCV2 (Swetha) 100 6.3 ± 0.88f 3.8 ± 0.78ef 4.8

1We used 100 embryo axis explants per treatment, and each treatment was repeated thrice.

2SE: standard error.

3Means ± SEs of values of three independent experiments. Values with the same letter within columns are not significantly different according to the DMRT5 at a 5% level.

4gus: β-glucuronidase.

5Transformation efficiency = (GUS explants/total explants infected) × 100.

6DMRT: Duncan’s multiple range test.

J Plant Biotechnol 2022;49:61-73 https://doi.org/10.5010/JPB.2022.49.1.061
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