Journal of Plant Biotechnology : eISSN 2384-1397 pISSN 1229-2818

Table. 1.

Table. 1.

Influence of co-cultivation duration and acetosyringone and cysteine concentrations on transformation efficiency in embryo axis explants of chickpea cv. JAKI92181

Co-cultivation duration (days) Acetosyringone concentration (µM/l) Cysteine concentration (mg/l) Explants infected, n gus2-Positive explants, mean ± SE3,4
0 - - 100 36.6 ± 1.47op
1 - - 100 38.2 ± 1.58op
2 - - 100 42.6 ± 1.63lm
3 - - 100 46.4 ± 1.74kl
4 - - 100 38.8 ± 1.68no
5 - - 100 36.4 ± 1.83op
3 0 - 100 46.4 ± 1.36kl
3 50 - 100 48.2 ± 1.65jk
3 100 - 100 66.2 ± 1.82cd
3 150 - 100 58.4 ± 1.68fg
3 200 - 100 56.2 ± 1.72gh
3 250 - 100 54.6 ± 1.66hi
3 100 0 100 66.2 ± 1.81cd
3 100 50 100 68.4 ± 1.65cd
3 100 100 100 70.6 ± 1.48cb
3 100 200 100 76.5 ± 1.60a
3 100 300 100 62.2 ± 1.78ef
3 100 400 100 60.8 ± 1.64ef

1The control group consisted of wounded explants precultured for 3 days at a bacterial cell density 1.0 at OD6005 and an infection duration of 30 min. Transformation efficiency = (GUS explants/total explants infected) × 100. We used 100 embryo axis explants per treatment, and each treatment was repeated thrice.

2gus: β-glucuronidase.

3SE: standard error.

4Means ± SEs of values of three independent experiments. Values with the same letter within columns are not significantly different according to the DMRT6 at a 5% level.

5OD600: optical density at 600 nm wavelength.

6DMRT: Duncan’s multiple range test.

J Plant Biotechnol 2022;49:61-73 https://doi.org/10.5010/JPB.2022.49.1.061
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