Journal of Plant Biotechnology : eISSN 2384-1397 pISSN 1229-2818

Table. 1.

Table. 1.

Effects of plant growth regulators on callus formation from root explant of Paeonia lactiflora Pall. cultured in Murashige and Skoog medium containing auxins and cytokinins in the dark for 6 weeks

Plant growth regulator (mg/L) Kinetin Zeatin BAP
0.0 0.5 1.0 2.0 0.5 1.0 2.0 0.5 1.0 2.0
IAA 0.5 +c + + + + + + + + +
1.0 + + + + + + + + + +
2.0 + + + + + + + + + +
3.0 + + + + + + + + + +
4.0 + + + + + + + + + +
NAA 0.5 + + + + ++ ++ + + + +
1.0 ++b ++ + + +++a ++++ ++ ++ + +
2.0 ++ ++ + + +++ +++ + ++ + +
3.0 + + + + + + + + + +
4.0 + + + + + + + + + +
IBA 0.5 + + + + + + + + + +
1.0 + + + + ++ ++ + + + +
2.0 + + + + + + + + + +
3.0 + + + + + + + + + +
4.0 + + + + + + + + + +
2,4-D 0.5 ++ ++ + + + + + + + +
1.0 ++ ++ ++ + ++ ++ ++ ++ + ++
2.0 +++ ++ + + ++ ++ ++ + + +
3.0 ++ + + + + + + + + +
4.0 + + + + + + + + + +

a>+++: very good callus formation, b++: good callus formation, c+: moderate callus formation

IAA: indole acetic acid, NAA, naphthalene acetic acid, IBA: indolebutyric acid, 2,4-D: 2,4-dichlorophenoxyacetic acid, BAP: benzylaminopurine
J Plant Biotechnol 2022;49:207-12 https://doi.org/10.5010/JPB.2022.49.3.207
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