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Screening of cryoprotectants (CPAs) for cryopreservation in the Nitzschia sp. of marine microalgae
J Plant Biotechnol 2018;45:400-408
Published online December 31, 2018
© 2018 The Korean Society for Plant Biotechnology.

In Hye Lee, Ji Young Jeon, Kyeung Mi Kim, Myung suk Kang

Biomaterials Research Team, National Institute of Biological Resources, Incheon 404-708, Republic of Korea
Correspondence to: e-mail:
Received September 21, 2018; Revised November 29, 2018; Accepted December 3, 2018.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Biodiversity has continued to degrade in the 21st century due to global warming occasioned by destruction of the environment around the world.. The Nagoya protocol places Korea in a unique position to effectively develop and protect its domestic genetic resources. Microalgae under study in this research contains large amount of antioxidant substances such as beta carotene and astaxanthin, that can be used as biological resource owing to the large amounts of biomass that can be secured through photosynthesis. However, it is difficult to preserve it since cryopreservation method used for long-term preservation is yet to be developed. A basic study for long term cryopreservation was carried out on Nizschia frustulum and Nitzschia amabilis which belong to marine diatoms. As cryoprotectants (CPAs), glycerol, DMSO, and methanol which penetrate into cells were prepared at 5%, 10%, and 15% concentrations each, in case of methanol, it was tested at concentrations of 5%, 10% and 12% by its nature. Two kinds of microalgae, N. frustulum and N. amabilis, were diluted with 102, 103 and 104 cells ml -1, respectively. The highest survival rate was shown at 12% concentration of methanol, and the figures were 6.94 ± 0.31% in N. frustulum and 8.85 ± 0.16% in N. amabilis. As a result of 3 weeks cultivation of thawed microalgae after freezing, the result is shows that N. frustulum increased about 10 times faster and N. amabilis increased about 12 times the original concentration.
Keywords : microalgae, diatom, cryoprotectants (CPAs)

December 2018, 45 (4)
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