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Genetic diversity assessment of lily genotypes native to Korea based on simple sequence repeat markers
J Plant Biotechnol 2019;46:158-164
Published online September 30, 2019
© 2019 The Korean Society for Plant Biotechnology.

Shipra Kumari†,#, · Young-Sun Kim · Bashistha Kumar Kanth · Ji-Young Jang · Geung-Joo Lee

Department of Horticultural Science, Chungnam National University, Daejeon 34134, Korea
Correspondence to: e-mail: gjlee@cnu.ac.kr

This authors contributed equally to this work.

#Current Address: Department of Agriculture Biotechnology, National Academy of Agriculture Science, Rural Development Administration, Jeonju, Jeollabuk-do 54874, Korea
Received June 16, 2019; Revised August 6, 2019; Accepted August 7, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Molecular characterization of different genotypes reveals accurate information about the degree of genetic diversity that helps to develop a proper breeding program. In this study, a total of 30 EST-based simple sequence repeat (EST-SSR) markers derived from trumpet lily (Lilium longiflorum) were used across 11 native lily species for their genetic relationship. Among these 30 markers, 24 SSR markers that showed polymorphism were used for evaluation of diversity spectrum. The allelic number at per locus ranged from 1 at SSR2 locus to 34 alleles at SSR15 locus, with an average of 11.25 alleles across 24 loci observed. The polymorphic information content, PIC, values ranged from 0.0523 for SSR9 to 0.9919 for SSR2 in all 24 loci with an average of 0.3827. The allelic frequency at every locus ranged from 0.81% at SSR2 locus to 99.6% at SSR14 locus. The pairwise genetic dissimilarity coefficient revealed the highest genetic distance with a value of 81.7% was in between L. dauricum and L. amabile. A relatively closer genetic distance was found between L. lancifolium and L. dauricum, L. maximowiczii and L. concolor, L. maximowiczii and L. distichum (Jeju), L. tsingtauense and L. callosum, L. cernuum and L. distichum (Jeju ecotype), of which dissimilarity coefficient was 50.0%. The molecular fingerprinting based on microsatellite marker could serve boldly to recognize genetically distant accessions and to sort morphologically close as well as duplicate accessions.
Keywords : EST-derived simple sequence repeats (EST-SSR), Genetic diversity, Native lily, Polymorphic information content (PIC).


September 2019, 46 (3)
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