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Relationship between ganglioside expression and anti-cancer effects of a plant-derived antibody in breast cancer cells
J Plant Biotechnol 2019;46:217-227
Published online September 30, 2019
© 2019 The Korean Society for Plant Biotechnology.

Won Seok Ju · Ilchan Song · Se-Ra Park · Sang Young Seo · Jin Hyoung Cho · Sung-Hun Min · Dae-Heon Kim· Ji-Su Kim· Sun-Uk Kim · Soon Ju Park · Kisung Ko · Young-Kug Choo

Department of Biological science, College of Natural Sciences, Wonkwang University, 460, Iksan-daero, Iksan-si, Jeollabuk-do 54538, Republic of Korea
Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University, 84, Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea
Center of Reproductive Medicine, Good Moonhwa Hospital, 119, Beomil-ro, Dong-gu, Busan 48735, Republic of Korea
Department of Biology, Sunchon National University, 235, Jungang-ro, Sunchon-si, Jeollanam-do 57922, Republic of Korea
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, 30, Yeonggudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si, Chungcheongbuk-do 28116, Republic of Korea
Institute for Glycoscience, Wonkwang University, 460, Iksan-daero, Iksan-si, Jeollabuk-do 54538, Republic of Korea
Correspondence to: e-mail: ykchoo@wku.ac.kr
Received August 29, 2019; Revised September 3, 2019; Accepted September 4, 2019.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Production of therapeutic monoclonal antibodies (mAbs) using a plant platform has been considered an alternative to the mammalian cell-based production system. A plant-derived mAb CO17-1AK (mAbP COK) can specifically bind to various types of cancer cell lines. The target protein of mAbP COK is the epithelial cell adhesion molecule (EpCAM) highly expressed in human epithelial cancer cells, including breast and colorectal cancer cells. It has been hypothesized that its overexpression supports tumor growth and metastasis. A ganglioside is extended well beyond the surfaces of the various cell membranes and has roles in cell growth, inflammation, differentiation, and carcinogenesis. However, the regulation of EpCAM gene expression in breast cancers and the role of gangliosides in oncogenesis are unclear. Here, the purpose of this study was to determine the effects of mAbP COK on human breast cancer cell proliferation, apoptosis, and ganglioside expression patterns. Our results show that treatment with mAbP COK suppressed the growth of breast cancer cells and induced apoptotic cell death. It also upregulated the expression of metastasis-related gangliosides in breast cancer cells. Thus, treatment with mAbP COK may have chemo-preventive therapeutic effects against human breast cancer.
Keywords : Anti-cancer, Apoptosis, Breast carcinoma, Ganglioside, Plant-derived antibody CO17-1A


September 2019, 46 (3)
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Funding Information
  • National Research Foundation of Korea
      10.13039/501100003725
      NRF-2017R1A4A1015594
  • Ministry of Science, ICT and Future Planning
      10.13039/501100003621
      2019R1F1A107
  • Korea Research Institute of Bioscience and Biotechnology
      10.13039/501100003715
      KGM4251824
  • System and Synthetic Agrobiotech Center
     
      PJ01342101
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