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Improvement of cadmium tolerance and accumulation of Phragmites spp. Tabarka by ethyl methane sulfonate mutagenesis
J Plant Biotechnol 2020;47:324-329
Published online December 31, 2020
© 2020 The Korean Society for Plant Biotechnology.

Young-Nam Kim・Jiseong Kim・Jeongeun Lee ・Sujung Kim・Keum-Ah Lee ・Sun-Hyung Kim

Department of Environmental Horticulture, University of Seoul, Seoul 02504, Republic of Korea
Correspondence to: e-mail: pgel2006@gmail.com
Received December 18, 2020; Revised December 22, 2020; Accepted December 22, 2020.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M2 mutant reeds under Cd stress conditions (up to 1,500 μM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.
Keywords : Ethyl methane sulfonate, Mutant reed, Cd tolerance, Hyperaccumulator, Phytoremediation


December 2020, 47 (4)
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