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Ex-situ conservation and cytotoxic activity assessment of native medicinal orchid: Coelogyne stricta
J Plant Biotechnol 2020;47:330-336
Published online December 31, 2020
© 2020 The Korean Society for Plant Biotechnology.

Bir Bahadur Thapa ・Laxmi Sen Thakuri ・Pusp Raj Joshi ・Krishna Chand ・Sabari Rajbahak ・Anil Kumar Sah ・ Resha Shrestha ・Mukti Ram Paudel ・So Young Park ・Bijaya Pant

Annapurna Research Center, Kathmandu 44618, Nepal
Central Department of Botany, Tribhuvan University, Kirtipur 44613 Kathmandu, Nepal
Department of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of Korea
Correspondence to: e-mail: b.pant@cdbtu.edu.np
Received July 20, 2020; Revised December 15, 2020; Accepted December 15, 2020.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.
Keywords : Coelogyne stricta, Conservation, HeLa cell line, Micropropagation, MTT assay


December 2020, 47 (4)
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  • KOICA/KU-Integrated Rural Development Project
     
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