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Induction of somatic embryogenesis from immature zygotic embryos and young apical leaves in cork oak (Quercus suber L.)
J Plant Biotechnol 2021;48:44-53
Published online March 31, 2021
© 2021 The Korean Society for Plant Biotechnology.

Zineb Nejjar El Ansari ·Brahim El Bouzdoudi ·Tomader Errabii ·Rabah Saidi ·Mohammed L’bachir El Kbiach

Biology Department, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco
Environmental Technologies, Biotechnology and Bio-resources Valorisation Team (ETBBV), Department of Biology, Faculty of Science and Technology Al Hoceima, Abdelmalek Essaadi University, Tetouan, Morocco
Sciences of Matter and Life Department, Tetouan Higher Teacher Training College of Tetouan, Abdelmalek Essaâdi University, Tetouan, Morocco
Correspondence to: e-mail: belbouzdoudi@uae.ac.ma
Received January 14, 2021; Revised March 24, 2021; Accepted March 24, 2021.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The present work aims to study the induction of somatic embryogenesis in cork oak (Quercus suber L.) from immature zygotic embryos and young apical leaves obtained from 2-month-old seedlings through acorn germination on sterilized peat. The immature zygotic embryos were grown for 1 month on the mineral solution of MS in the presence of 4.52 μM 2,4-D and 30 g/L sucrose. They were then transferred to the same mineral solution with no added growth regulators. In the third subculture, yellow somatic embryos, characterized by two voluminous cotyledons, were differentiated from the radicle of the immature zygotic embryos. The induction of somatic embryogenesis in young leaves required a series of transfers on different culture media containing 30 g/L sucrose and 100 mg/L myo-inositol. Secondary or recurrent somatic embryogenesis occurred within the immature somatic embryo radicles after 1 month of culture on growth regulator-free medium containing WPM macronutrients, MS micronutrients, and vitamins.
Keywords : Cork oak, Quercus suber L., Immature zygotic embryos, Young apical leaves, Somatic embryogenesis


March 2021, 48 (1)
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