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Development of molecular markers for the differentiation of Angelica gigas Jiri line by using ARMS-PCR analysis
J Plant Biotechnol 2021;48:26-33
Published online March 31, 2021
© 2021 The Korean Society for Plant Biotechnology.

Shin-Woo Lee ·Soo Jin Lee ·Eun-Hee Han ·Yong-Wook Shin ·Yun-Hee Kim

Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University, JinJu, Korea
Gyeongsangnam-do Agricultural Research and Extension Services, Jinju 52733, Korea
Department of Biology Education, College of Education, IALS, Gyeongsang National University, Jinju, Korea
Correspondence to: e-mail: cefle@gnu.ac.kr
Received February 4, 2021; Revised March 6, 2021; Accepted March 23, 2021.
cc This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Angelica is a widely used medicinal and perennial plant. Information on the genetic diversity of Angelica populations is essential for their conservation and germ plasmic utilization. Although Angelica is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish it from other similar species from different countries. This developed single nucleotide polymorphism (SNP) markers derived from nuclear ribosomal DNA internal transcribed spacer regions genomic sequences to identify distinct Korean-specific Angelica species via amplification refractory mutation system (ARMS)-PCR curve analyses. We performed molecular authentication of different kinds of Korean-specific Angelica species such as A. gigas Nakai and A. gigas Jiri using DNA sequences in the ITS intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific Angelica species from different countr.
Keywords : Angelica, ARMS-PCR, Nuclear ribosomal DNA internal transcribed spacer regions, Single nucleotide polymorphisms


March 2021, 48 (1)
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